Project & Microscope Requests

Steps to Access Cryo-EM

Contact SLAC Regional Cryo-EM Consortium

Email Us:
wahc@stanford.edu

Upcoming Events

Regional Cryo-EM Consortium event:
PI Retreat September 28, 2019 8:30 AM - 12:00 PM

Instructions for requesting Regional Cryo-EM Consortium resources:

1. Microscope Time Request

Approved Regional Cryo-EM Consortium members should request on-site or remote time to collect images on samples by submitting a Microscope Time Request through the User Portal. Sample details and variations from the original project proposal are to be included in each Microscope Time Request.

A call for requests is made on a quarterly basis and the management team will make allocation determinations after Microscope Time Requests have been reviewed for safety and hazardous material concerns by SLAC/Stanford staff.

Questions regarding Microscope Time Request submittal can be directed to the Cryo-EM User Office.

2. Communication Prior to Data Collection: Our staff will communicate with users a week or two before scheduled microscope time to aid with logistics and answer any questions you may have.

3. Sample Shipment to and from SLAC: Users will ship the frozen grid to SLAC one week prior to the date of data collection. The sample and the dry shipper will be sent back to the users after one week of the data collection.  We can archive the frozen grid during the period of the project. Shipping instructions

4. Remote Access: Secured remote access to operate the cryo-electron microscopes is available. Users who wish to make use of this must apply for a SLAC unix account and indicate remote data collection a part of the the Microscope Time Request. Further details here.

5. Data Transfer: As soon as your experiment has started, your experimental data will be made available on our central data storage. A SLAC unix account is required to access the data. Standard unix tools like scp and rsync are available, as is Globus. Further details here.

6. Data Storage and Analysis: SLAC will provide a short term (3 months) storage on disk and long term (3 years) on magnetic tape. On the fly data analysis to evaluate image quality is available. The more extensive computing resources required for 3-D reconstruction are available through a competitive application process.

7. User Agreement: The Stanford-SLAC cryo-EM facilities are located on the premises of a Department of Energy laboratory site. As such, a Cryo-EM User Agreement or addendum to an existing DOE User Agreement covering experiments at the SSRL and the LCLS must be executed before the beginning of the experiment. Once executed the agreement will cover cryo-EM experiments for any group from that institution. The list of institutions that have User Agreements with SLAC can be found here.