S²C² Cryo-EM Facilities

Coming to SLAC for Cryo-EM

 Information for Visitor/User/Employee

Contacts

General Questions:

s2c2@slac.stanford.edu

Contacts for Cryo-EM Services

 

Arrillaga Science Center Microscope Image Gallery

Facilities

S2C2 is equipped with state-of-the-art instruments including three 300 kV electron microscopes (Alpha, Beta and Gamma) for high-resolution data collection. In addition, we have two other electron microscopes for training and specimen screening (TEM2 and TEM3). Cryo-specimen preparation devices and cell tissue culture/biochemical labs are also on premises.

S2C2 Cryo-Electron Microscopes Configuration Table

Microscope TEM2 TEM3 TEM Alpha** TEM Beta** TEM Gamma

Model

Titan Krios G3 Talos Arctica G2 Titan Krios G3i Titan Krios G3i Titan Krios G3i
kV 300 200 300 300 300
Detector K3 K2 Falcon 4 K3 K3

Energy Filter

BioQuantum BioQuantum N/A BioQuantum BioQuantum
Data Collection Software

EPU 2.5

Tomo 4.10

SerialEM 3.8

EPU 2.5

TOMO 4.10

SerialEM 3.8

EPU 2.7

TOMO 5.2

EPU 2.7

TOMO 4.12

EPU 2.7

TOMO 4.12

Volta Phase Plate Yes Yes Yes Yes Yes
Users Multiple Users Multiple Users S²C² S²C² S²C²
Fringe Free Not Available Not Available Yes Future Update Future Update
Apoferritin Structure Acheivable *1.72Å Not Available 1.36Å 1.34Å Not Available

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

*Collected on K2 prior to K3 upgrade

**Nominal Maginifications on TEM Alpha and TEM Beta

 

S2C2 Arrillaga Science Building

 

 

Data Management and Computational Resources

All experimental data collected under S2C2 will be held on disk for two months from the day of collection. The data is transferred as soon as it is collected and is therefore immediately available for download via either the usual unix tools (rsync, scp, bbcp, etc) or through globus. More details regarding data transfer can be found at https://confluence.slac.stanford.edu/x/mYoYDg.

After two months, the data is then moved onto tape and will be kept for 22 additional months. Requests for data retrieval after the data is moved onto tape can be made via email to unix-admin@slac.stanford.edu with specific information regarding the date of the data collection, the proposal number and the microscope that was used.

 Two years after the data is collected, the data will be purged completely from our systems.

 As part of our data services, all collected movie images will be automatically aligned (motion corrected) and CTF calculations performed and reported.