Frequently Asked Questions (FAQs)

  • Does TEM Alpha support EER format?

    Answer:

    Yes, we have added this format for efficient file storage with preservation of spatial and temporal resolution.

  • Why is my CTF correction on all images above a certain value?

    Answer:

    This may be a possible result of thick ice and particle distribution.

  • Can you recover and return my grids with the dewar?

    Answer:

    Yes, we can.

  • Can you store my unused grids for a later session

    Answer:

    Yes, we have dewar storage space.

  • Can EPU collect data on lacey grids?

    Answer:

    Yes, the latest version of EPU is also optimized for lacey grids.

  • How many images can we expect per hour - and therefore, per two-day session?

    Answer:

    This depends on your data acquisition magnification, Quantifoil hole size, sample and whether stage tilt is used.

  • What is the nominal magnification for my requested pixel size?

    Answer:

    Please see below link for table and also, there is a table at every TEM from which the operator reads this value.

    Link:  Nominal Magnifications on TEM Alpha and TEM Beta

  • How many grids should we send?

    Answer:

    Always send a least duplicates, and preferably four grids of each sample. Since grid quality varies even during a single plunge freezing session, and also when repeating identical protocols at different sessions, we prefer to receive multiple grids of each sample, and then decide on the optimal grid after doing an Atlas of all grids.

  • How many grids can be imaged in a day/session?

    Answer:

    This depends on the quality of the grid, and the number of grid squared and Quantifoil holes that can be used for data collection.  We always aim to choose the best grid on which most data can be collected, which often results in a grid per day, and often one grid will be used for the total two day session.  Users may also request one grid per day, when their grids are from different specimens.

  • Who do I contact if I have questions?

    Answer:

    All contacts for Cryo-EM services can be found here:  Contacts

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