Frequently Asked Questions (FAQs)

  • Does Falcon 4 on TEM beta have an MTF file to use in Relion?

    Answer:

    Yes, we can send that to you.

  • What is the spherical aberration value for the Krios?

    Answer:

    Cs value is 2.7

  • Does TEM Alpha support EER format?

    Answer:

    Yes, we have added this format for efficient file storage with preservation of spatial and temporal resolution.

  • Why is my CTF correction on all images above a certain value?

    Answer:

    Thick ice and particle distribution may affect CTF value.

  • Can you recover and return my grids with the dewar?

    Answer:

    Yes, we can.  See our Project Application Page for more information regarding this question.

  • Can you store my unused grids for a later session

    Answer:

    Yes, we have dewar storage space.

  • Can EPU collect data on lacey grids?

    Answer:

    Yes, the latest version of EPU is also optimized for lacey grids.

  • How many images can we expect per hour - and therefore, per two-day session?

    Answer:

    This depends on your data acquisition magnification, Quantifoil hole size, sample and whether stage tilt is used.

  • What is the nominal magnification for my requested pixel size?

    Answer:

    Please see below link for table and also, there is a table at every TEM from which the operator reads this value.

    Link:  Nominal Magnifications on TEM Alpha and TEM Beta

  • How many grids should we send?

    Answer:

    Always send at least duplicates, and preferably four grids of each sample. Since grid quality varies even during a single plunge freezing session, and also when repeating identical protocols at different sessions, we prefer to receive multiple grids of each sample, and then decide on the optimal grid after doing an Atlas of all grids.

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