Frequently Asked Questions (FAQs)

  • Where can I find more information on cryo-EM data storage and transfer at S2C2​?

    Answer:

    Please refer to this site for more information on SLAC Shared Scientific Data Facility.

  • Does Falcon 4 on TEM beta have an MTF file to use in Relion?

    Answer:

    Yes, we can send that to you.

  • What is the spherical aberration value for the Krios?

    Answer:

    Cs value is 2.7

  • Does TEM Alpha support EER format?

    Answer:

    Yes, we have added this format for efficient file storage with preservation of spatial and temporal resolution.

  • Why is my CTF correction on all images above a certain value?

    Answer:

    Thick ice and particle distribution may affect CTF value.

  • Can you recover and return my grids with the dewar?

    Answer:

    Yes, we can.  See our Project Application Page for more information regarding this question.

  • Can you store my unused grids for a later session

    Answer:

    Yes, we have dewar storage space.

  • Can EPU collect data on lacey grids?

    Answer:

    Yes, the latest version of EPU is also optimized for lacey grids.

  • How many images can we expect per hour - and therefore, per two-day session?

    Answer:

    This depends on your data acquisition magnification, Quantifoil hole size, sample and whether stage tilt is used.

  • What is the nominal magnification for my requested pixel size?

    Answer:

    Please see below link for table and also, there is a table at every TEM from which the operator reads this value.

    Link:  Nominal Magnifications on TEM Alpha and TEM Beta

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